Metagenomics
Over 99% of natural microorganisms can not be isolated and cultured clonally. Traditional isolation and culture-dependent methods have limited the study of microorganisms in their natural environment. Metagenomics studies of genetic material that is directly recovered from environmental samples, have benefitted greatly from advanced NGS technology as a method for the exploration of microbial biodiversity NGC offers whole genome shotgun sequencing of DNA isolated from environmental samples, with theadvantages of high throughput and high coverage. It can provide information not only on species composition and abundance, but also on functional genes, gene differences between samples, metabolic pathways and gene resource mining for bioactive products.
We at National Genomics Core-CDFD have meticulous quality control in each step of the sequencing project. Experts with PhD and International experience are using top-notch technologies to carefully plan and generate high quality, meaningful data.
Over 99% of natural microorganisms can not be isolated and cultured clonally. Traditional isolation and culture-dependent methods have limited the study of microorganisms in their natural environment. Metagenomics studies of genetic material that is directly recovered from environmental samples, have benefitted greatly from advanced NGS technology as a method for the exploration of microbial biodiversity.
16S and 18S rDNA are hypervariable regions in the 16S or 18S rRNA genes in bacteria and fungi, while ITS (Internal Transcribed Spacer) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi and archaea. Sequence comparison of 16S/18S/ITS regions is widely used in taxonomy and molecular phylogeny because of the easy amplification by PCR, even from low quantities of DNA, while they have a high degree of variation even between closely related species.
We at National Genomics Core-CDFD have meticulous quality control in each step of the sequencing project. Experts with PhD and International experience are using top-notch technologies to carefully plan and generate high quality, meaningful data.
