Epigenomics
Sodium bisulfite conversion of genomic DNA to differentiate and detect unmethylated versus methylated cytosines is the gold standard for DNA methylation analysis. NGS based sequencing of bisulphite treated DNA provides single nucleotide resolution map of 5-methyl cytosine of the genome. Bisulfite conversion sequencing can be done with targeted methods such as amplicon methyl-seq or target enrichment, or with whole-genome bisulfite sequencing.
We at National Genomics Core-CDFD have meticulous quality control in each step of the sequencing project. Experts with PhD and International experience are using top-notch technologies to carefully plan and generate high quality, meaningful data
ChIP Sequencing is widely used to analyze protein interactions with DNA. It combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins, and can be used to precisely map global binding sites for any protein of interest. ChIP sequencing offers higher resolution and more precise and abundant information in comparison with array-based ChIP-chip.
We at National Genomics Core-CDFD have meticulous quality control in each step of the sequencing project. Experts with PhD and International experience are using top-notch technologies to carefully plan and generate high quality, meaningful data.
